Scrub Typhus IgG/IgM Rapid Test Cassette

【SUMMARY】
Scrub typhus or bush typhus is a form of typhus caused by the intracellular parasite Orientia tsutsugamushi, a Gram-negative α-proteobacterium of family Rickettsiaceae first isolated and identified in 1930 in Japan. Although the disease is similar in presentation to other forms of typhus, its pathogen is no longer included in genus Rickettsia with the typhus bacteria proper, but in Orientia. The disease is thus frequently classified separately from the other typhi. Signs and symptoms include fever, headache, muscle pain, cough, and gastrointestinal symptoms. More virulent strains of O. Tsutsugamushi can cause hemorrhaging and intravascular coagulation. Morbilliform rash, eschar, splenomegaly, and lymphadenopathies are typical signs. Leukopenia and abnormal liver function tests are commonly seen in the early phase of the illness. Pneumonitis, encephalitis, and myocarditis occur in the late phase of illness. It has particularly been shown to be the most common cause of acute encephalitis syndrome in Bihar, India.

【DIRECTIONS FOR USE】
Allow test cassette, specimen, buffer and/or controls to equilibrate to room temperature (15-30℃) prior to testing.
1. Bring the pouch to room temperature before opening. Remove the test cassette from the sealed pouch and use it within one hour.
2. Place the test cassette on a clean and level surface.
· For Serum or Plasma Specimens:
Ø To use a dropper: Hold the dropper vertically, draw the specimen up to the Fill Line (approximately 10μl), and transfer the specimen to the sample well of the test cassette, then add 2 drops of buffer (approximately 80ul) and start the timer. Avoid trapping air bubbles in the specimen well.
Ø To use a micropipette: Pipette and dispense 10µl of specimen to the sample well of the test cassette, then add 2 drops of buffer (approximately 80 µl) and start the timer.
· For Whole Blood (Venipuncture/Fingerstick) Specimens:
Ø To use a dropper: Hold the dropper vertically, draw the specimen about 1cm above the Fill Line, and transfer 1 full drop of whole blood (approximately 20 µl) to the sample well of the test cassette, then add 2 drops of buffer (approximately 80 ul) and start the timer.
 To use a micropipette: Pipette and dispense 20 µl of whole blood to the specimen well of the test cassette, then add 2 drops of buffer (approximately 80 µl) and start the timer.
3. Wait for the colored line(s) to appear. The test result should be read at 15 minutes. Do not interpret the result after 20 minutes.
Note: It is suggested not to use the buffer, beyond 6 months after opening the vial.