FOB Rapid Test Dipstick

Many diseases can cause hidden blood in the feces. This is also known as Fecal Occult Blood (FOB), Human Occult Blood, or Human Hemoglobin. In the early stages,gastrointestinal problems such as colon cancer, ulcers, polyps, colitis, diverticulitis, andfissures may not show any visible symptoms, only occult blood. Traditional guaiac-basedmethods lack sensitivity and specificity, and also have diet restrictions prior totesting.

The FOB Rapid Test Dipstick (Feces) is a rapid test toqualitatively detect low levels of Fecal Occult Blood. The test uses a double antibodysandwich assay to selectively detect Fecal Occult Blood at 40ng/ml or higher, or 4.8μg/g feces. In addition, unlike guaiac assays, the accuracy of the test is not affected bythe diet of the patients.

 

【DIRECTIONS FOR USE】

Allow the test, specimen, buffer and/or controls to reach room temperature (15- 30°C) prior to testing.1. To collect fecal specimens:

Collect sufficient quantity of feces (1-2 mL or 1-2 g) in a clean, dry specimen collection container to obtain maximum antigens (if present). Best results will be obtained if the assay is performed within 6 hours after collection. Specimen collected may be stored for 3 days at 2-8℃ if not tested within 6 hours. For long term storage, specimens should be kept below -20℃.

2. To process fecal specimens:

• For Solid Specimens:

Unscrew the cap of the specimen collection tube，then randomly stab the specimen collection applicator into the fecal specimen in at least 3 different sites to collect approximately 50 mg of feces (equivalent to 1/4 of a pea). Do not scoop the fecal specimen.

• For Liquid Specimens:

Hold the dropper vertically, aspirate fecal specimens, and then transfer 2 drops (approximately 80 μL) into the specimen collection tube containing the extraction buffer.

3. Bring the pouch to room temperature before opening it. Remove the test dipstick from the foil pouch and use it as soon as possible. Best results will be obtained if the test is performed immediately after opening the foil pouch.

4. Fix the reaction tube, hold the specimen collection tube upright and break off the tip of the specimen collection tube. Invert the specimen collection tube and transfer 8- 10 full drops of the extracted specimen (approx. 500μL) to the reaction tube, then with arrows pointing toward the exaction buffer, immerse the dipstick and start the timer. Do not immerse the dipstick past the maximum line. See illustration below.

5. Read results at 5 minutes. Do not read results after 10 minutes.