Plasmid nucleic acid extraction kit (magnetic bead method) adopts magnetic bead and buffer system with unique separation function, combines magnetic nano-separation technology with SDS alkaline lysis method of bacterial cells, releases nucleic acid in the buffer system, and under the effect of centrifugal force The next cell debris and SDS complexes settle down. Add special coated magnetic beads, the magnetic beads have a strong affinity for plasmid DNA in the buffer system, and when the conditions change, the magnetic beads release the adsorbed nucleic acids, and the washing system can remove impurities such as proteins and small molecules in the solution. , can achieve the purpose of rapid separation and purification of nucleic acid, and does not use toxic reagents such as chloroform.
Application:
Widely used in scientific research, hospital, biological industry, etc.
Feature:
1. Simple and fast: Ultrapure plasmid DNA can be obtained in about 45 minutes
2. High purity: effectively remove impurities such as protein and inorganic salts, and the product A260/280 value is greater than 1.7
3. Good quality: with a unique buffer, it can release DNA better and improve the yield, and it also has little damage to genomic DNA, which can protect the integrity of DNA
4. Automation: match BNP32, BNP48, BNP96 nucleic acid extractors to achieve high-throughput automated operation
5. Safe and non-toxic: the reagent does not contain toxic solvents such as phenol and chloroform
6. Wide range of applications: enzyme digestion, PCR, library construction, Southern hybridization, etc.
Technical Parameter:
Model |
CH-13-1 |
CH-13 -2 |
CH-13-3 |
Extraction Method |
Magnetic bead method |
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Sample Type |
Bacterial liquid |
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Validity Period |
Good stability, valid for 12 months |
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Sample Volume |
1~2ml |
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Within-assay Precision |
Coefficient of variation (CV,%)≤15% |
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Specification |
8T/box,16T/box, 32T/box, 64T/box |
48T/box, 96T/box |
50T/box, 100T/box |
Applicable Instruments |
BNP32, BNP48 |
BNP96 |
Manual Extraction |
Package Information |
24 Boxes/Carton |
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Package Size(W*D*H) |
740*420*300mm |
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Gross Weight |
18.5kg |